Enany, M., Wahdan, A., Al-Ghobary, M., Hassan, W., Abo Hashem, M. (2021). Bacterial Causes of Hemorrhagic Gastroenteritis in Dogs and Cats with Detection of Some Virulence and β-lactamase Resistance Genes in Escherichia coli and Salmonella by Multiplex PCR. Suez Canal Veterinary Medical Journal. SCVMJ, 26(1), 39-59. doi: 10.21608/scvmj.2021.184734
Mohamed Enany; Ali Wahdan; Marwa Al-Ghobary; Wafaa M Hassan; Marwa Abo Hashem. "Bacterial Causes of Hemorrhagic Gastroenteritis in Dogs and Cats with Detection of Some Virulence and β-lactamase Resistance Genes in Escherichia coli and Salmonella by Multiplex PCR". Suez Canal Veterinary Medical Journal. SCVMJ, 26, 1, 2021, 39-59. doi: 10.21608/scvmj.2021.184734
Enany, M., Wahdan, A., Al-Ghobary, M., Hassan, W., Abo Hashem, M. (2021). 'Bacterial Causes of Hemorrhagic Gastroenteritis in Dogs and Cats with Detection of Some Virulence and β-lactamase Resistance Genes in Escherichia coli and Salmonella by Multiplex PCR', Suez Canal Veterinary Medical Journal. SCVMJ, 26(1), pp. 39-59. doi: 10.21608/scvmj.2021.184734
Enany, M., Wahdan, A., Al-Ghobary, M., Hassan, W., Abo Hashem, M. Bacterial Causes of Hemorrhagic Gastroenteritis in Dogs and Cats with Detection of Some Virulence and β-lactamase Resistance Genes in Escherichia coli and Salmonella by Multiplex PCR. Suez Canal Veterinary Medical Journal. SCVMJ, 2021; 26(1): 39-59. doi: 10.21608/scvmj.2021.184734
Bacterial Causes of Hemorrhagic Gastroenteritis in Dogs and Cats with Detection of Some Virulence and β-lactamase Resistance Genes in Escherichia coli and Salmonella by Multiplex PCR
1Department of Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia Governorate, Egypt.
2Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, 41522 Ismailia, Egypt.
3Department of Zoonotic Diseases, Veterinary Medicine Directorate, Damietta
4Microbiology, Reference lab. of Quality Control on Poultry Production, Animal Health Research Institute
5Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicie, Suez Canal University, Ismailia, Egypt.
Abstract
Hemorrhagic Gastroenteritis (HGE) is a life-threatening disease caused by bacteria or virus or endoparasites or irritant drugs or food allergy. Out of 202 sampled animals,the total bacterial isolates were 104. The identified bacterial isolates were 46 (44.23%) E. coli, 9 (8.65%) Klebsiella, 5 (4.80%) C. perfringens, 22 (21.15%) Proteus spp., 3 (2.88%) Salmonella spp., 3 (2.88%) Shigella spp. and 4 (3.84%) Pseudomonas aeruginosa, 4 (3.84%) Enterobacter species, 2 (1.92%) Citrobacter species, 2 (1.92%) Providencia rettgeri, 1 (0.96%) Hafnia species, 1 (0.96%) Serratia liquefaciens, 1 (0.96%) C. bifermentans and 1 (0.96%) C. putrefaciens. EHEC (12/26, 46.15%), EPEC (9/26, 34.61%) and ETEC (4/26, 15.38%) strains were detected by E. coli serotyping. Salmonella isolates were serotyped as Salmonella Typhimurium, S. Heidelberg and S. Infantis. E. coli isolates from dogs were resistant to amoxicillin/clavulanic acid, cephalexin, ceftriaxone, trimethoprim/sulphamethoxazole, tetracycline, and erythromycin. Feline E. coli isolates had moderate resistance to amoxicillin/clavulanic acid, trimethoprim/ sulphonamides, and tetracycline. Salmonella isolates were highly resistant to amoxicillin/clavulanic acid, cephalexin, trimethoprim/sulphamethoxazole, tetracycline, and erythromycin. stx1 and stx2 E. coli virulence genes were detected in 80% and 60% of tested E. coli isolates, respectively while S. Typhimurium was positive for invA, hilA and fimH virulence genes and S. Heidelberg was positive for invA and fimH genes. blaTEM and blaCTX-M1 β-lactamase resistance genes were detected in 60% and 20% of tested E. coli isolates, respectively. Salmonella Typhimuriumwas positive for blaCMY-1 and blaOXA-2 genes, Salmonella Heidelberg was positive for blaCMY-1 gene. In conclusion, E. coli, Salmonella, C. perfringens, Klebsiella were major bacterial causes of HGE in dogs and cats. Additionally, E. coli and Salmonella isolated from companion animals can carry multidrug resistance genes encoding for extended-spectrum β-lactamases.
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