A worst-case scenario for nosocomial infections led to the rise of vancomycin-resistant enterococci (VRE). This study assesses the occurrence of Enterococcus faecalis in 200 fecal samples and 133 milk samples collected from cattle and buffalo. About 70 human samples were collected as stool samples and hand swabs from animal clinics and farms. Isolation and identification of the bacteria were performed using conventional cultural techniques, and biochemical identification and were confirmed by PCR amplification. Antimicrobial susceptibility against six antimicrobial agents commonly used in humans and animals was tested using the disc diffusion method. The resistance of E. faecalis to vancomycin (VAN) was confirmed by PCR targeting the vanA and vanB genes. The occurrence of E. faecalis in cattle and buffaloes was 8.1% and 15.5% respectively. Normal milk samples showed 12.8% of E. faecalis while abnormal milk samples were 37.5%. E. faecalis infection was recorded at 25.7% in workers’ contact. Antimicrobial-resistant to Vancomycin was 11.1%,14.2%, 10.5%, and 50% in cattle, buffalo, milk, and human respectively. Resistance genes (van A and van B) were detected in all Vancomycin-resistant strains. This study showed that E. faecalis which is vancomycin-resistant can be discovered not only in milk and animal products but also in human contact, which is a matter of public health
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