Hepatoprotective Effects of Moringa Oleifera Seed Oil and Pumpkin Seed Oil on CCL4 Induced Hepatotoxicity in Rats

Abdalla, Osama A. M.; Hashem, Mohamed A.; Abdelnaeim, Noha S.; Elazouney, Doaa M. S.

doi:10.21608/scvmj.2022.281309

Hepatoprotective Effects of Moringa Oleifera Seed Oil and Pumpkin Seed Oil on CCL4 Induced Hepatotoxicity in Rats

Document Type : Original Article

Authors

¹ Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal university, 41522, Egypt

² Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, 44519, Egypt

³ Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal University, 41522, Egypt

10.21608/scvmj.2022.281309

Abstract

This study was designed to estimate the hepatoprotective and antioxidant effects of Moringa oleifera seed oil (MOSO) and pumpkin seed oil (PSO) against the hepatic toxicity induced by CCL₄ in rats. Sixty male albino rats (120 ± 10 g) were randomly divided into 6 equal groups. Group 1 was the control group and was orally given corn oil once daily for 28 days. Group 2 was given MOSO at a dose of 0.2 ml/rat, while group 3 received PSO (300 mg/kg b. wt.) once daily for 28 days. Group 4 received CCL₄ i/p at a dose of 2.5 ml/kg b. wt. on days 13 and 14 of the experiment and was designated as a positive control group. Groups 5, and 6 received MOSO and PSO at the same dose, route, and duration as groups 2 and 3 and were injected with CCL₄ i/p at a dose of 2.5 ml/kg b. wt. on days 13 and 14 of the experiment. The results showed that oral administration of MOSO and PSO to CCL₄-intoxicated rats on day 28 of the experiment significantly reduced the elevated levels of liver enzymes and bilirubin and elevated serum levels of total protein, albumin, and globulin, as well as a significant improved in lipid profile compared to the CCL₄-intoxicated group. Furthermore, GSH and CAT levels were significantly higher in the treated groups, while MDA levels were significantly lower compared to the CCL₄ intoxicated group. The histopathological examination of liver sections in rats pretreated with MOSO and PSO showed favorable changes in the degenerative changes induced by CCL₄. Our results propose that oral administration of Moringa oleifera and pumpkin seed oils have potent antioxidant and hepatoprotective effects against CCL₄ toxicity in rats.

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