Molecular Characterization and Multidrug Resistance of Yersinia Species in Fish

Document Type : Original Article

Authors

1 1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University

2 Department of Bacteriology, Animal Health Research Institute (AHRI), Mansoura lab. Agriculture Research Center (ARC).

3 Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University

4 Free veterinarian

Abstract

A total of, (100) samples of O. Niloticus and (100) samples of C. Gariepinus were gathered from multiple areas in Dakahlia governorate from April 2019 to April 20. Fish samples were subjected to clinical and microbiological examination from the kidney, liver, and spleen. Isolates were characterized by cultural characters, some biochemical tests, Api 20 system, and PCR. 24 diseased fish were characterized as infected with Y. ruckeri [10 from 100] O. niloticus (10%) and 14 from C. Gariepinus (14%)] with 44 Yersinia ruckeri isolates with the percentage of 22%. 16srRNA gene (specific common gene) was demonstrated in all Y.ruckeri isolates by PCR in addition to the detection of virulence genes (yrp,yrIlm,yhlA,yhlB,yrInv) in them by PCR assay and multi-drug resistance genes (blaTEM,qnrS, tetAgene). The study results showed that PCR is a rapid and reliable method for Y. ruckeri isolates identification which will be useful in the prevention and control of Yersiniosis.

Keywords

Main Subjects

Files

Share

How to cite

Statistics