Khafagy, A., Esawy, A., Wahdan, A., El feky, T., El-Deeb, S., Abo Hashem, M. (2023). Molecular Characterization and Multidrug Resistance of Yersinia Species in Fish. Suez Canal Veterinary Medical Journal. SCVMJ, 28(1), 1-15. doi: 10.21608/scvmj.2023.289215
Ahmed Khafagy; Abo Elkheir Esawy; Ali Wahdan; Tamer El feky; Sohila El-Deeb; Marwa Abo Hashem. "Molecular Characterization and Multidrug Resistance of Yersinia Species in Fish". Suez Canal Veterinary Medical Journal. SCVMJ, 28, 1, 2023, 1-15. doi: 10.21608/scvmj.2023.289215
Khafagy, A., Esawy, A., Wahdan, A., El feky, T., El-Deeb, S., Abo Hashem, M. (2023). 'Molecular Characterization and Multidrug Resistance of Yersinia Species in Fish', Suez Canal Veterinary Medical Journal. SCVMJ, 28(1), pp. 1-15. doi: 10.21608/scvmj.2023.289215
Khafagy, A., Esawy, A., Wahdan, A., El feky, T., El-Deeb, S., Abo Hashem, M. Molecular Characterization and Multidrug Resistance of Yersinia Species in Fish. Suez Canal Veterinary Medical Journal. SCVMJ, 2023; 28(1): 1-15. doi: 10.21608/scvmj.2023.289215
Molecular Characterization and Multidrug Resistance of Yersinia Species in Fish
11Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University
2Department of Bacteriology, Animal Health Research Institute (AHRI), Mansoura lab. Agriculture Research Center (ARC).
3Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University
4Free veterinarian
Abstract
A total of, (100) samples of O. Niloticus and (100) samples of C. Gariepinus were gathered from multiple areas in Dakahlia governorate from April 2019 to April 20. Fish samples were subjected to clinical and microbiological examination from the kidney, liver, and spleen. Isolates were characterized by cultural characters, some biochemical tests, Api 20 system, and PCR. 24 diseased fish were characterized as infected with Y. ruckeri [10 from 100] O. niloticus (10%) and 14 from C. Gariepinus (14%)] with 44 Yersinia ruckeri isolates with the percentage of 22%. 16srRNA gene (specific common gene) was demonstrated in all Y.ruckeri isolates by PCR in addition to the detection of virulence genes (yrp,yrIlm,yhlA,yhlB,yrInv) in them by PCR assay and multi-drug resistance genes (blaTEM,qnrS, tetAgene). The study results showed that PCR is a rapid and reliable method for Y. ruckeri isolates identification which will be useful in the prevention and control of Yersiniosis.
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