Comparative study between traditional methods, commercial biochemical test and PCR for identification of Campylobacter species isolated from poultry farms

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Document Type : Original Article

Authors

1 Animal Health Research Institute, Mansoura Lab. Agriculture Research Center (ARC)

2 Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Suez Canal University

3 Department of Microbiology, Animal Reproduction Research Institute

4 Animal Health Research Institute (AHRI), Mansoura lab. Agricultural Research Center (ARC), Egypt

Abstract

Campylobacter spp. causes severe issues in chickens and human being. In this investigation. Campylobacter species were isolated (182) in Dakahlia Governorate's from 440 broilers and 440 ballade breeds. Campylobacter jejuni (16%) and Campylobacter coli (4%). For C. jejuni, the rates of recovery from various internal organs were 60%, 24%, 10%, and 5%, from the cloacal swab, heart, liver, and gizzard. . The morphological studies revealed that the majority of Campylobacter species are motile, grow at 37-42Co, and are G-ve, slender spirally curved. They require 5%O2, 10%CO2, and 85%N2 to create a colony that looks like a dew drop on mCCDA media. By application of biochemical tests and API20 assay ,the suspected isolates were identical to typical Campylobacter spp. and by application PCR assay was carried out for(c.coli and c.jejuni ) to detect 23srRNA as common gene for campylobacter SPP , and hipO gene for C.jejuni and asp gene for C .Coli

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Suez Canal Veterinary Medical Journal. SCVMJ

Articles in Press, Accepted Manuscript
Available Online from 08 March 2023

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