Al Qabili, D., Aboueisha, A., Youssef, A., El-Mahallawy, H. (2023). Virulence and Antimicrobial resistance of Shiga Toxin‑producing E. coli (STEC) isolated from Tilapia, Mullet and Its Public Health Significance. Suez Canal Veterinary Medical Journal. SCVMJ, (), -. doi: 10.21608/scvmj.2023.186626.1113
Dheyazan M. Ali Al Qabili; Abdelkarim Aboueisha; Ahmed Ibrahim Youssef; Heba S. El-Mahallawy. "Virulence and Antimicrobial resistance of Shiga Toxin‑producing E. coli (STEC) isolated from Tilapia, Mullet and Its Public Health Significance". Suez Canal Veterinary Medical Journal. SCVMJ, , , 2023, -. doi: 10.21608/scvmj.2023.186626.1113
Al Qabili, D., Aboueisha, A., Youssef, A., El-Mahallawy, H. (2023). 'Virulence and Antimicrobial resistance of Shiga Toxin‑producing E. coli (STEC) isolated from Tilapia, Mullet and Its Public Health Significance', Suez Canal Veterinary Medical Journal. SCVMJ, (), pp. -. doi: 10.21608/scvmj.2023.186626.1113
Al Qabili, D., Aboueisha, A., Youssef, A., El-Mahallawy, H. Virulence and Antimicrobial resistance of Shiga Toxin‑producing E. coli (STEC) isolated from Tilapia, Mullet and Its Public Health Significance. Suez Canal Veterinary Medical Journal. SCVMJ, 2023; (): -. doi: 10.21608/scvmj.2023.186626.1113
Virulence and Antimicrobial resistance of Shiga Toxin‑producing E. coli (STEC) isolated from Tilapia, Mullet and Its Public Health Significance
Articles in Press, Accepted Manuscript, Available Online from 12 March 2023
1Agriculture and Veterinary Medicine College, Thamar University, Republic of Yemen
2Department of Zoonoses, Faculty of Veterinary Medicine- Suez Canal University
3Department Of Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
4Department of Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Abstract
Shiga Toxin‑producing E. coli (STEC) are responsible for numerous food-borne outbreaks worldwide. In this study, tissues samples of finfish (tilapia, n = 100) and (mullet, n = 100), and 20 human hand swab samples from fishermen and fish sellers were examined bacteriologically for the presence of STEC. Isolates were tested for their antibiotic sensitivity and examined for detection of stx1, stx2 and eaeA. Overall, E. coli and STEC isolates were identified from the tissues (36.5% and 12.5%) of the examined tilapia and mullet, respectively, however, the prevalence of E. coli and STEC from human hand swabs was as high as (60% and 40%), respectively. Among the total of E. coli isolates, 25 presumptive STEC isolates (17 from finfish and 8 isolate in human) showed characteristic mauve colonies on CHROMagar STEC medium. The highest prevalence of STEC was in mullet and tilapia from fresh water of Nile tributaries 24% and 48%, followed by fish from fresh water fish farms by 16% and 12%, respectively. No STEC were isolated from fish from Suez Canal water and saltwater fish farms. Recovered STEC isolates from fish belonged to 14 serotypes belonging to (O121:H7, O113:H4, O128:H2, O119:H6, O146:H2, O153:H2, O91:H21, O26:H11, O44:H18, O124, O159, O78, O55:H7 and O117: H4). Isolates from human hand swabs belonged to (O26:H11, O121:H7, O91:H21, O15:H2, and O119:H6). One or two shiga-toxin (stx1 and stx2) genes were confirmed in STEC isolates. STEC isolates from finfish and humans were multi-drug resistant (MDR). The current study revealed a high level of contamination of finfish from Ismailia governorate with STEC and highlighted the high level of antimicrobial resistance exhibited which is very hazardous to the consumers.
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