Genetic traits of Some Gastrointestinal Bacteria Isolated from Dorcas Gazelles Collection at Giza Zoo

Othman, Nahed; Kamel, Atef; Khafagy, Ahmed; Saad, Enas Mohamed

doi:10.21608/scvmj.2023.209755.1131

Genetic traits of Some Gastrointestinal Bacteria Isolated from Dorcas Gazelles Collection at Giza Zoo

Articles in Press

Document Type : Original Article

Authors

¹ Wildlife and Zoo Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt

² Professor of wildlife and zoo Vice dean of community service and Environmental department faculty of veterinary medicine Ain Shams University

³ Professor of Bacteriology, Mycologyand ImmunologyDean of faculty of Veterinary MedicineAin shams University

⁴ Lecturer of Wildlife and zooFaculty of VeterinarySuez Canal University

10.21608/scvmj.2023.209755.1131

Abstract

The Dorcas gazelles (Gazella Dorcas) are strict herbivorous which play an important role in the ecological balance. They are breed in zoos and susceptible to various bacterial affections such as Escherichia coli, Salmonella and Klebsiella, causing them as a source of diseases for other animals and human. The present study aimed to investigate the gastrointestinal bacteria in the population of Dorcas gazelles at Giza Zoo at different seasons of the year and identifying their antibiotic sensitivity, virulence, and resistance traits with PCR. A total of 70 fecal swabs were collected and examined. The yielded E. coli was the most dominant bacteria followed by Klebsiella then Salmonella and others. The isolated bacteria showed high multiple resistance with various degrees to erythromycin, tetracycline, ceftazidime, clindamycin and cefaclor. The fimH and invA virulence genes were detected in the isolated E. coli and Salmonella isolates respectively. Moreover, the molecular examination of antibiotic resistant genes was confirmed the presence of blaCTX-M and tetA in all isolates of E. coli and Salmonella. To our knowledge, this is the first study which reports the fecal shedding of these zoonotic bacteria from Dorcas gazelles in Egypt. Further studies are required to evaluate the other bacterial burden infecting this vulnerable species.

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