Comparative Study Between Traditional Methods, Commercial Biochemical Test and PCR for Identification of Campylobacter Species Isolated from Poultry Farms

Document Type : Original Article

Authors

1 Bacteriology, Immunology and Mycology Department, Faculty of Veterinary medicine, Suez Canal University

2 Animal Health Research Institute, Mansoura Lab. Agriculture Research Center (ARC)

3 Animal Reproduction Research institute.

Abstract

Campylobacter spp < /em>. causes severe issues in chickens. In this investigation. Campylobacter species were isolated (182) in Dakahlia Governorate from 440 broilers and 440 ballade breeds.   Campylobacter coli 42 isolates(4%)and Campylobacter jejuni140isolates (16%). For C. jejuni, rates of recovery from various internal organs were 60%, 24%, 10%, and 5%, from the cloacal swab, heart, liver, and gizzard respectively. The morphological studies revealed that the majority of species from Campylobacter are motile, survive at 37-42Co, and are G-ve, slender spirally curled. They require 5%O2, 85%N2, and 10%CO2 to create a colony that looks like a dew drop on mCCDA media. By using API20, the suspected isolates were identical to typical Campylobacter

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