Khafagy, A., Kamel, A., abouelgheit, S., Abu-Elala, N., Eidaros, N., abdelrazeq, F. (2023). Bacteriological Studies on Sea Turtles in Suez Governorate. Suez Canal Veterinary Medical Journal. SCVMJ, 28(2), 485-494. doi: 10.21608/scvmj.2024.241450.1149
Ahmed Khafagy; Atef Mohamed Kamel; Sayed abouelgheit; nermeen Abu-Elala; Nada Eidaros; fatma elzahraa abdelrazeq. "Bacteriological Studies on Sea Turtles in Suez Governorate". Suez Canal Veterinary Medical Journal. SCVMJ, 28, 2, 2023, 485-494. doi: 10.21608/scvmj.2024.241450.1149
Khafagy, A., Kamel, A., abouelgheit, S., Abu-Elala, N., Eidaros, N., abdelrazeq, F. (2023). 'Bacteriological Studies on Sea Turtles in Suez Governorate', Suez Canal Veterinary Medical Journal. SCVMJ, 28(2), pp. 485-494. doi: 10.21608/scvmj.2024.241450.1149
Khafagy, A., Kamel, A., abouelgheit, S., Abu-Elala, N., Eidaros, N., abdelrazeq, F. Bacteriological Studies on Sea Turtles in Suez Governorate. Suez Canal Veterinary Medical Journal. SCVMJ, 2023; 28(2): 485-494. doi: 10.21608/scvmj.2024.241450.1149
Bacteriological Studies on Sea Turtles in Suez Governorate
1Professor of Microbiology, Faculty of Veterinary Medicine, Suez Canal University
2Wildlife and Zoo Medicine Dept., Faculty of Vet. Med., Suez Canal University
3professor of micribiology at National Institute of oceanography and fisheries
4Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt
5Dep. of Bacteriology, Faculty of Veterinary Medicine
6bacteriology,suez canal university
Abstract
Sea turtles are air-breathing, marine reptiles. The past few years have witnessed a surge in research focusing on the causes of morbidity and mortality, along with intensified endeavors to conserve them. This research aimed to isolate and characterize vibrio alginolyticus from apparently healthy sea turtles, Antimicrobial sensitivity test, and Molecular identification. There were 60 red-eared turtle samples (30 buccal and 30 cloacal samples), and 18 green turtle samples (9 buccal and 9 cloacal samples). collected aseptically from the Red Sea, Pet stores, and Giza zoo and subjected to isolation and biochemical characterization of V. alginolyticu, V. alginolyticus are Gram-negative motile comma-shaped rodes that are facultatively anaerobic. Isolates of V. alginolyticus are divided into (9) positive isolates from Red-eared turtles, and (3) from Green turtles. All tested isolates were positive for catalase, oxidase, indole test, Methyl red, and citrate utilization and Negative for Vogus prosper. The V. alginolyticus strain exhibited major resistance to trimethoprim 5µg, ampicillin10µg, streptomycin 10µg based on the antibiotic sensitivity test, besides the intermediate sensitivity to Tobramycin 10µg, Naldixic acid 30µg and kanamycin (30µg). Furthermore, the bacterium demonstrated high sensitivity to Chloramphenicol 30µg, Tetracycline 30µg, and Ciprofloxacin 5µg. The molecular identification was achieved by utilizing species-specific primers to target the collagenase gene.
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