Molecular Characterization of S. Aureus Isolated from Cases of Bovine Mastitis

Document Type : Original Article

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1 Department of Bacteriology, Immunology and Mycology - Faculty of vet. medicine - Ain shamsl university

2 Department of Bacteriology, Immunology and Mycology - Faculty of vet. medicine - Suez canal university

Abstract

The purpose of the investigation was to find S. aureus that had been isolated from raw cow's milk. 152 milk samples (42 clinical mastitis and 110 apparently healthy milk) were collected from 152 dairy cows from different regions at Damietta Governorate. Subclinical mastitis was found using the California mastitis test. Prevalence of mastitis was 59% (90/152). Moreover, the prevalence of clinical mastitis was 21.1% (32/152) and subclinical mastitis 48.3% (58/120). To isolate bacteria, a loopful of suspicious milk was cultivated on various culture media. Following biochemical testing, PCR, and an examination of culture characteristics on several selective media, the isolated bacteria were identified. Fifty of the 152 samples tested positive for S. aureus. The isolated S. aureus was verified by PCR. The information gathered for this study helps to clarify how common S. aureus is in the Damietta Governorate as a cause of mastitis.

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