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Eid, H., Shalaby, A., Shtewy, M. (2017). Detection of Virulence Genes of Staphylococcus Aureus Isolated from Chicken Using Polymerase Chain Reaction.. Suez Canal Veterinary Medical Journal. SCVMJ, 22(1), 19-29. doi: 10.21608/scvmj.2017.62375
Hamza Eid; Amany Shalaby; Mera Shtewy. "Detection of Virulence Genes of Staphylococcus Aureus Isolated from Chicken Using Polymerase Chain Reaction.". Suez Canal Veterinary Medical Journal. SCVMJ, 22, 1, 2017, 19-29. doi: 10.21608/scvmj.2017.62375
Eid, H., Shalaby, A., Shtewy, M. (2017). 'Detection of Virulence Genes of Staphylococcus Aureus Isolated from Chicken Using Polymerase Chain Reaction.', Suez Canal Veterinary Medical Journal. SCVMJ, 22(1), pp. 19-29. doi: 10.21608/scvmj.2017.62375
Eid, H., Shalaby, A., Shtewy, M. Detection of Virulence Genes of Staphylococcus Aureus Isolated from Chicken Using Polymerase Chain Reaction.. Suez Canal Veterinary Medical Journal. SCVMJ, 2017; 22(1): 19-29. doi: 10.21608/scvmj.2017.62375

Detection of Virulence Genes of Staphylococcus Aureus Isolated from Chicken Using Polymerase Chain Reaction.

Article 2, Volume 22, Issue 1, June 2017, Page 19-29  XML PDF (749.1 K)
Document Type: Original Article
DOI: 10.21608/scvmj.2017.62375
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Authors
Hamza Eid1; Amany Shalaby2; Mera Shtewy* 3
1Department of Microbiology, Faculty of Veterinary Medicine, Suez Canal University.
2Department of Food Hygiene, Animal Health Research Institute, Port Said Branch
3Veterinarian
Abstract
A total of 300 chicken samples (100 samples for breast muscles, 100 samples for thigh muscles and 100 samples for joints) were collected randomly from different markets in Port-said city during the period of work from July 2015 till August 2016. Overall percentage of coagulase positive S. aureus which isolated from chicken samples were 37.2% (49/132) while coagulase positive S. aureus of breast muscles, thigh muscles and joints were 15.1%, 14.5% and 7.6% respectively. PCR revealed that 15 tested isolates were S. aureus using 16SrRNA at 791 bp. The study conducted to detect virulence genes, coa 15 isolates were positive at 570 bp. (100%),  clfA15 isolates were positive at 638 bp. (100%),  See gene is the highest SE, 5 isolates were positive at 209 bp. (33.33%), followed by Seb gene 1isolate were positive at164 bp. (6.67%). None of 15 isolates were positive for Sea, Sec and Sed.
Keywords
Staphylococcus aureus; Detection of Virulence Genes; chicken; polymerase chain reaction
Main Subjects
Bacteriology, Immunology and Mycology
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