Multiplex PCR of Some Antibiotic Resistance Genes of Methicillin Resistant Staphylococcus Aureus (MRSA) Isolated from Infected Cat Fish (Clarias garipeneaus).

Document Type : Original Article

Authors

1 Dept. of Microbiology Faculty of Vet. Medicine, Suez Canal University, Ismailia, Egypt.

2 Central laboratory for Aquaculture Research, Agriculture Research Center, Egypt

3 Department of Microbiology, Faculty of Vet. Medicine, Suez Canal University, Ismailia, Egypt.

Abstract

 This study was designed to detect some antibiotic resistance genes of S.aureus by using PCR. A total of 150 Cat fish (Clarias garipeneaus) with a body weight ranged from200 – 250g.  suffered from ulcerated skin and erosion. S. aureus were isolated in percent of 100% from infected fish by using Baird parker as selective media. By using multiplex PCR for investigation some antibiotic resistant genes, as erm A gen,  aac (6)-aph (2) genes , van A gen and  mec A gene. All isolated S. aureus were sensitive to Amikacin, Amoxicillin, Ampicillin and Ciprofloxacin while resistant to Gentamicin, Methicillin and Neomycin.

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