Sidr honey inhibitory effect on virulence genes of MRSA strains from animal and human origin

Enany, Mohamed; AL-Gammal, A.; Hanora, A.; Shagar, G.; El Shaffy, N.

doi:10.21608/scvmj.2015.64562

Sidr honey inhibitory effect on virulence genes of MRSA strains from animal and human origin

Document Type : Original Article

Authors

¹ Dept. of Microbiology, Faculty of Vet. Med. Suez Canal Univ.

² Dept. of Microbiology, Faculty of Vet. Med. Suez Canal Univ

³ Dept. of Microbiology and immunology, Fac. of Pharmacy. Suez Canal Univ.

⁴ Dept. of Fish health and care, central lab. For fisheries research.AL Abassa. Egypt.

⁵ Dept. of Fish health and care, central lab. For fisheries research.AL Abassa. Egypt

10.21608/scvmj.2015.64562

Abstract

The effect of sidr honey on the expression of meca, coa and spa genes and their antibacterial activities was assayed against MRSA strains isolated from 200 clinically mastitic milk samples and170 samples (45wounds, 30 sputum, 25 blood, 30aspirates, 20 urine and 20 cerebrospinal fluids) were collected from hospitals in Sharkia Governorate. Five different sidr honey samples from {Egypt (E), Libya ( L) ,Yemen (Y), Pakistan (P) and Saudi Arabia (S) } were used in this study . After the bacteriological examination of the collected samples as well as the susceptibility to methicillin , the percentage of MRSA strains was found to be 35.5 %. MRSA strains. Meca, coa and spa genes were detected by PCR before and after the exposure to sidr honey . The absence of ( meca, coa and spa) gnes in MRSA strains after exposure to sidr honey was noticed. Briefly, sidr honey has an inhibitory effect on mec, coa and spa genes of MRSA.

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