Eid, H., Nasef, S., Hassonna, N. (2015). Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry. Suez Canal Veterinary Medical Journal. SCVMJ, 20(2), 175-189. doi: 10.21608/scvmj.2015.64626
Hamza Eid; Soad Nasef; Nosaiba Hassonna. "Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry". Suez Canal Veterinary Medical Journal. SCVMJ, 20, 2, 2015, 175-189. doi: 10.21608/scvmj.2015.64626
Eid, H., Nasef, S., Hassonna, N. (2015). 'Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry', Suez Canal Veterinary Medical Journal. SCVMJ, 20(2), pp. 175-189. doi: 10.21608/scvmj.2015.64626
Eid, H., Nasef, S., Hassonna, N. Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry. Suez Canal Veterinary Medical Journal. SCVMJ, 2015; 20(2): 175-189. doi: 10.21608/scvmj.2015.64626
Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry
1Dept. of Bacteriology, Mycology and Immunology, Fac. of Vet. Medicine, Suez Canal Univ., Egypt
2Dept. of Bacteriology,Mycology and Immunology, Fac. of Vet. Medicine, Suez Canal Univ., Egypt
3Animal Health Research Institute , Dokki, Egypt
Abstract
Three hundred samples of infected and freshly dead chickens from different farms gathered in Hehia city El-sharkia for bacteriological examination to detect the presence of Salmonella, 23 Salmonella isolates were detected from 300 samples.Serological results detected Salmonella enteritidis with percentage 26.1 % , followed by Salmonella typhimurium with percentage 17.4 % and Salmonella kentucky 8.7% . All Salmonella isolates were tested for their susceptibility to the following antimicrobial agents: gentamicin(CN), ,ciprofloxacin (CIP), amoxicillin- clavulanic acid (AMC), , doxycyclin (DO), chloramphenicol (C), erythromycin (E), sulfamethoxazole -trimethoprim (SXT) High rate of susceptibility was the most common finding obtained against ciprofloxacin (75%) as shown in table (24). Also, absolute resistance was obtained among Salmonella isolates against erythromycin (100%) and amoxicillin clavulanic acid (100%), and sulfamethoxazole -trimethoprim (25%).In addition, 33.3% of isolates were resistant to chloramphenicol, and colistin sulfate. All isolates were resistant to at least three antibiotics and multidrug resistance was seen .PCR detected 6 types of antibiotic resistant genes (aadB gene, qnrS gene ,sul1 gene , floR gene ,dfrA gene and blaTEM gene) in percentage 91.7% ,83.3% ,66.7%, 75%,33.3% and 83.3% respectively .