Prevalence and Molecular Characterization of Vibrio Spp. in Fish and Shellfish from Port Said Coastal Area

Eid, Hamza; Soliman, Zainab; Hanafy, Al-Shaimaa

doi:10.21608/scvmj.2015.64641

Prevalence and Molecular Characterization of Vibrio Spp. in Fish and Shellfish from Port Said Coastal Area

Document Type : Original Article

Authors

¹ Department of Bacteriology, Immunology, Mycology - Faculty of Veterinary Medicine, Suez Canal University.

² Animal Health Research Institute-El-Dokki.

³ Department of Bacteriology, Animal Health Research Institute, Port Said Branch

10.21608/scvmj.2015.64641

Abstract

This study was carried out during the period from April 2014 to November 2014. A total of 250 seafood samples, 50 samples each namely Mullet, Sardine, Shrimp, Cuttlefish and Mussel, obtained by random sampling from the coast of Port Said Governorate. Samples were subjected to conventional method for the detection of Vibrio species. Overall, 32% of the Mullet, 36% of Sardine, 52% of Shrimp, 44% of both Cuttlefish and Mussel samples were found to be positive for Vibrio spp < /em>. The prevalence of Vibrio species was higher in shellfish samples, particularly in shrimp than that of fish samples. Four Vibrio species were identified. V. alginolyticus was found to be the dominant identified Vibrio species with total prevalence of 48.2% (50/104) followed by V. damsela 24% (25/104), V. harveyi 16.3% (17/104) and V. parahaemolyticus 11.5% (12/104). Eight isolates identified phenotypically as Vibrio species, were confirmed by PCR.

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