Phenotypic Identification of Clostridium Perfringens Associated with Necrotic Enteritis of Broiler Chickens in Sharkia Governorate

Enany, Mohamed; Ammar, Ahmed; Maghwery, Mona; Gamal., Noha

doi:10.21608/scvmj.2015.64648

Phenotypic Identification of Clostridium Perfringens Associated with Necrotic Enteritis of Broiler Chickens in Sharkia Governorate

Document Type : Original Article

Authors

¹ Bacteriology, Immunology and Mycology Department, Fac. of Vet. Med., Suez Canal University

² Bacteriology, Mycology and Immunology Department, Fac. of Vet. Med., Zagazig University

³ Anaerobic Unit, Bacteriology Research Department, Animal Health Research Institute, Dokki, Giza.

10.21608/scvmj.2015.64648

Abstract

A total of 314 samples of liver and intestinal loop were collected from broiler chickens suffering from diarrhea during 2013-2014 .The tissue samples were examined for the prevelance of C.perfringens revealed 91 (66.93%) of them were toxinogenic and 44(31.06%) non toxinogenic isolates .One hundered and thirty two samples were positive for Clostridium perfringens typing by intradermal inoculation in guinea pig. The higher incidence of C.perfringens wasrecorded in winter (53.12%-57.5%) followed by autumn (50%) then summer (32.60%-37.93%) and the lower incidence was recorded in spring (30%-31.25%).The incidence of C.perfringens from intestinal samples was(65.85%-58.66%)and liver samples were(21.95%-21.33%). The most effective antibiotic was recommended for treatment of necrotic enteritis were amoxicillin, Ampicillin, Piperacillin, ceftraxione, cefruxime, Cefotaxime which was applied by sensitivity test to 91 isolates of toxinogenic Clostridium perfringens. Our study recorded that there is the isolation rate of Clostridium perfringens from intestine was exteremly higher than liver samples and ranged from (58.66%-65.85%) and (21.95 %-21.33), respectively that subtyped into type A 76 (57.57%) and typeD 15 (11.36%).

Main Subjects