Saad, E., Kamel, A., Khafagy, A., Gamal Eldein, M. (2015). Serological and Molecular Studies on Multi-drug Resistant Salmonella Isolated from Captive Budgerigars (Melopsittacus undulatus). Suez Canal Veterinary Medical Journal. SCVMJ, 20(1), 135-145. doi: 10.21608/scvmj.2015.65028
Enas Saad; Atef Kamel; Ahmed Khafagy; Mohamed Gamal Eldein. "Serological and Molecular Studies on Multi-drug Resistant Salmonella Isolated from Captive Budgerigars (Melopsittacus undulatus)". Suez Canal Veterinary Medical Journal. SCVMJ, 20, 1, 2015, 135-145. doi: 10.21608/scvmj.2015.65028
Saad, E., Kamel, A., Khafagy, A., Gamal Eldein, M. (2015). 'Serological and Molecular Studies on Multi-drug Resistant Salmonella Isolated from Captive Budgerigars (Melopsittacus undulatus)', Suez Canal Veterinary Medical Journal. SCVMJ, 20(1), pp. 135-145. doi: 10.21608/scvmj.2015.65028
Saad, E., Kamel, A., Khafagy, A., Gamal Eldein, M. Serological and Molecular Studies on Multi-drug Resistant Salmonella Isolated from Captive Budgerigars (Melopsittacus undulatus). Suez Canal Veterinary Medical Journal. SCVMJ, 2015; 20(1): 135-145. doi: 10.21608/scvmj.2015.65028
Serological and Molecular Studies on Multi-drug Resistant Salmonella Isolated from Captive Budgerigars (Melopsittacus undulatus)
1Wildlife Management and Zoo Medicine Dept. Faculty of Veterinary Medicine. Suez Canal University.
2Microbiology Dept. , Faculty of Veterinary Medicine. Suez Canal University.
Abstract
The present work was carried out for serological and molecular screening of virulence genes associated with Salmonella in captive budgerigars. A total of 805 apparently healthy birds were collected from different sources, and subjected to clinical and bacteriological examination. Salmonella species were isolated at rate of (4.97%) with recognition of 4 different serovars in which Salmonella Paratyphi A was the most common isolated serotype. All isolates were highly sensitive to Ciprofloxacin, Enrofloxacin and Norfloxacin. Multiplex-PCR using (invA, spvC and stn) was devised to confirm the isolates and predict their virulence. (invA;284bp) was detected in all Salmonella isolates with (100%), while (stn;617bp)and (spvC; 392bp) were detected in some Salmonella isolates.
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