Serological and Molecular Studies on Multi-drug Resistant Salmonella Isolated from Captive Budgerigars (Melopsittacus undulatus)

Document Type : Original Article

Authors

1 Wildlife Management and Zoo Medicine Dept. Faculty of Veterinary Medicine. Suez Canal University.

2 Microbiology Dept. , Faculty of Veterinary Medicine. Suez Canal University.

Abstract

The present work was carried out for serological and molecular screening of virulence genes associated with Salmonella in captive budgerigars. A total of 805 apparently healthy birds were collected from different sources, and subjected to clinical and bacteriological examination. Salmonella species were isolated at rate of (4.97%) with recognition of 4 different serovars in which Salmonella Paratyphi A was the most common isolated serotype. All isolates were highly sensitive to Ciprofloxacin, Enrofloxacin and Norfloxacin. Multiplex-PCR using (invA, spvC and stn) was devised to confirm the isolates and predict their virulence. (invA;284bp) was detected in all Salmonella isolates with (100%), while (stn;617bp)and (spvC; 392bp) were detected in some Salmonella isolates.

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