Saad, E., Kamel, A., Khafagy, A., Gamal Eldein, M. (2015). Diagnostic Investigation of Salmonella in Captive Budgerigars Using Standard Microbiological Techniques and invA Gene Specific PCR. Suez Canal Veterinary Medical Journal. SCVMJ, 20(1), 147-156. doi: 10.21608/scvmj.2015.65029
Enas Saad; Atef Kamel; Ahmed Khafagy; Mohamed Gamal Eldein. "Diagnostic Investigation of Salmonella in Captive Budgerigars Using Standard Microbiological Techniques and invA Gene Specific PCR". Suez Canal Veterinary Medical Journal. SCVMJ, 20, 1, 2015, 147-156. doi: 10.21608/scvmj.2015.65029
Saad, E., Kamel, A., Khafagy, A., Gamal Eldein, M. (2015). 'Diagnostic Investigation of Salmonella in Captive Budgerigars Using Standard Microbiological Techniques and invA Gene Specific PCR', Suez Canal Veterinary Medical Journal. SCVMJ, 20(1), pp. 147-156. doi: 10.21608/scvmj.2015.65029
Saad, E., Kamel, A., Khafagy, A., Gamal Eldein, M. Diagnostic Investigation of Salmonella in Captive Budgerigars Using Standard Microbiological Techniques and invA Gene Specific PCR. Suez Canal Veterinary Medical Journal. SCVMJ, 2015; 20(1): 147-156. doi: 10.21608/scvmj.2015.65029
Diagnostic Investigation of Salmonella in Captive Budgerigars Using Standard Microbiological Techniques and invA Gene Specific PCR
1Wildlife Management and Zoo Medicine Dept. ,Faculty of Veterinary Medicine. Suez Canal University.
2Microbiology Dept. ,Faculty of Veterinary Medicine. Suez Canal University.
Abstract
A total of 384 faecal samples were collected from apparently healthy budgerigars and processed to investigate the status of Salmonella in captive budgerigars by Standard Microbiological Techniques (SMT) and PCR with invA gene. (10.42%) were Salmonella positive by SMT while (18.49%) were positive by PCR. The incidence by SMT was (11.31%, 12.50% and 6.25%) while, by PCR (25%, 16.67% and 9.38%) in zoos, pet shops and household groups, respectively. The sensitivity and specificity of the PCR were (100% and 91%) respectively. Individual samples that examined by SMT and PCR were compared with Pooling method which considered as initial screening method that eliminates all Salmonella-negative samples. Enriched faecal broth and PCR with invA gene can be used as rapid method for direct detection of Salmonella in the faecal samples of carrier captive birds. Salmonella isolation rate was (87.5%, 7.5%, 5% and 0%) in Spring, Summer, Winter and Autumn respectively.
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