Detection of antibiotic resistant genes of some Campylobacter species isolated from Egyptian ducks

Hamed, Engy; Abdel Rahman, Mona; Shalaby, Azhar; Morsy, Mai; Nasef, Soad

doi:10.21608/scvmj.2014.65801

Detection of antibiotic resistant genes of some Campylobacter species isolated from Egyptian ducks

Document Type : Original Article

Authors

National Laboratory for Quality Control on Poultry production, Animal Health Research Institute, Ministry of Agriculture, Nady El-Seid Street, Dokki P.O. Box246, Giza 12618, Egypt

10.21608/scvmj.2014.65801

Abstract

Campylobactercoli and Campylobacter jejuni may cause gastrointestinal disorders with or without necrotic hepatitis in poultry and serious foodborne enteritis with sometimes fatal consequences in humans. Little is known about the prevalence of Campylobacter spp. in ducks, particularly young ducklings.In this study, 36 (24%) isolates of Campylobacterspp. were isolated from 150 samples of 1-day-old ducklings in Egypt. Using biochemical tests and specific PCR, 33 C. coli and 3 C. jejuni were identified. Allisolates were sensitive to chloramphenicol and amikacin but resistant to sulfonamethoxazole-trimethoprim (SXT) using antibiotic disc-diffusion test. The majority of isolates were susceptible to tetracycline and erythromycin, meanwhile the resistance to ofloxacin and ciprofloxacin was relatively high. Nine out of 33 C. coli were positive for the tetracycline resistance gene tet (O), although only two out of them were resistant to tetracycline. A polymorphism in the quinolone resistance-determining region (QRDR) of gyrA gene from C. coliand C. jejuni isolates was identiﬁed by direct sequencing. These findings indicated that ducklings may be a source for antibiotic resistant Campylobacter spp. with potential poultry and public health hazards.

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