Molecular characterization of Listeria monocytogenes isolated from milk

Khaphagy, Ahmed; Salama, S.; Ibrahim, A.; Hamouda, R.

doi:10.21608/scvmj.2014.76090

Molecular characterization of Listeria monocytogenes isolated from milk

Document Type : Original Article

Authors

¹ Dept. of Bacteriology, Immunology and Mycology, Faculty of Vet. Med. Suez Canal University.

² Central Laboratory for Evalution of Veterinary biologics, Abbasia, Cairo

³ Animal Reproductive Research Institute in Giza

10.21608/scvmj.2014.76090

Abstract

A total of 560 various raw milk samples were collected from different dairy farms to examine the presence of L.monocytogenes. These isolates were further characterized by biochemical tests and Polymerase Chain Reaction (PCR). L.monocytogenes was isolated in 72 of the 560 samples (12.9%). Based on PCR, All the examined L.monocytogenes were positive for Listeriolysin O gene (hly A) and P60 (iap).The findings of this study show that consumption of raw milk could be a potential risk of human listeriosis.

Keywords

Main Subjects