Enany, M., Younes, S., AL gammal, A., Salem, M., El Dieb, H. (2013). Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis. Suez Canal Veterinary Medical Journal. SCVMJ, 18(1), 139-147. doi: 10.21608/scvmj.2013.78287
Mohamed Enany; Soha Younes; Abd Elazeem AL gammal; Mohamed Salem; Hoda El Dieb. "Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis". Suez Canal Veterinary Medical Journal. SCVMJ, 18, 1, 2013, 139-147. doi: 10.21608/scvmj.2013.78287
Enany, M., Younes, S., AL gammal, A., Salem, M., El Dieb, H. (2013). 'Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis', Suez Canal Veterinary Medical Journal. SCVMJ, 18(1), pp. 139-147. doi: 10.21608/scvmj.2013.78287
Enany, M., Younes, S., AL gammal, A., Salem, M., El Dieb, H. Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis. Suez Canal Veterinary Medical Journal. SCVMJ, 2013; 18(1): 139-147. doi: 10.21608/scvmj.2013.78287
Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis
1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University
2Department of Clinical pathology, Faculty of Medicine, Suez Canal University
3Department of Animal Medicine, Faculty of Veterinary Medicine, Suez Canal University
Abstract
In order to determine the prevalence of S. aureus in clinical and subclinical bovine mastitis as well as the relationship between the presence of protein A gene and the udder inflammatory response . A total of 400 quarter milk samples were collected from 100 lactating Friesian cows at Ismailia Governorate . The percentage of clinical mastitis was ( 19.5%.), while the percentage of subclinical mastitis according to C.M.T was ( 42.85%) . The percentage of S.aureus in clinical mastitis was (28.2%) , while in in subclinical mastitis was (22.46% ).The identification of S,aureus was confirmed by PCR amplification of species-specific parts of the gene encoding the 16S rRNA. Twenty nine S.aureus isolates were subjected to PCR for detection of X region of protein A (spA) gene,10 isolates from clinical mastitis and 19 isolates subclinical mastitiC milk specimens (+++ve CMT and ++ve CMT) were (100%) positive . Results indicate that there was a positive relationship between the presence of protein A gene of S.aureus and the inflammation of udder in clinical mastitis as well as the high grades of subclinical mastitis.
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