Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis

Document Type : Original Article

Authors

1 Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University

2 Department of Clinical pathology, Faculty of Medicine, Suez Canal University

3 Department of Animal Medicine, Faculty of Veterinary Medicine, Suez Canal University

Abstract

In order to determine the prevalence of S. aureus in clinical and subclinical bovine mastitis as well as the relationship between the presence of protein A gene and the udder inflammatory response .   A total of 400 quarter milk samples were collected from 100 lactating Friesian cows  at Ismailia Governorate . The percentage of clinical mastitis was   ( 19.5%.), while the percentage of subclinical mastitis according to C.M.T was ( 42.85%) . The percentage of  S.aureus   in clinical  mastitis was (28.2%) , while in  in subclinical mastitis  was (22.46% ).The identification of S,aureus was confirmed by PCR amplification of species-specific parts of the gene encoding the 16S rRNA. Twenty nine  S.aureus isolates were subjected to PCR for detection of X region of protein A (spA) gene,10 isolates from clinical mastitis and 19  isolates subclinical mastitiC milk specimens (+++ve CMT and ++ve CMT)  were (100%) positive . Results indicate that there  was a positive relationship between the  presence of protein A gene of S.aureus and the inflammation of udder in clinical mastitis as well as the high grades of subclinical mastitis. 

Keywords

Main Subjects

Files

Share

How to cite

Statistics