Hematological Findings Associated with the Use of Licorice and Silymarin in Cisplatin Treated Rats

Abdelhamed, Randa Mahmod; Abdalla, Osama Mohamed; ELkilany, Omnia ELsaid

doi:10.21608/scvmj.2022.144716.1086

Hematological Findings Associated with the Use of Licorice and Silymarin in Cisplatin Treated Rats

Articles in Press

Document Type : Original Article

Authors

¹ clinical pathology,veterinary medecine faculty,suez canal university

² Professor of Clinical Pathology Faculty of Veterinary Medicine, Suez Canal University

³ faculty of veterinary medecine department of clinical pathology

10.21608/scvmj.2022.144716.1086

Abstract

The purpose of this study was to assess the effect of licorice and silymarin supplementations on some hematological parameters in cisplatin treated albino rats.
A total of 72 normal male albino rats’ weight 150 -200 gm. The rats were allowed for acclimation for one week at the animal shelter. The rats were divided randomly into six groups, each with 12 rats. Group (1): normal control group and fed with commercial balanced diet. Group (2): licorice extract orally treated group for 6 weeks at a rate of 2 gm/kg body weight. Group (3): silymarin orally treated group at a rate of 100mg/kg every day for six weeks. Group (4): served as hepatotoxic group treated by injection of cisplatin (CP) intraperitoneally in a dose of 1.5 mg/kg body weight every two weeks for three weeks starting from the 4^th week of the experiment. Group (5): received licorice extract orally treated group at a rate of 2 gm/kg body weight for 6 weeks. and injected cisplatin intraperitoneally in a dose of 1.5 g/kg body weight repeated twice a week for 6 weeks starting from the 4^th week of the experiment. Group (6): received silymarin orally treated group at a rate of 100mg/kg daily for 6 weeks. and injected cisplatin intraperitoneally in a dose of 1.5 mg/kg body weight every two weeks for six weeks starting from the 4^th week of the experiment.
The purpose of this study was to investigate the effects of oral administration of licorice and silymarin extracts for 6 weeks on some hematological parameters in cisplatin treated albino rats. These effects were explored by measuring hematological parameters.
Group injected with cisplatin revealed decrease RBCs count, Hb concentration, and values of PCV, MCV, MCH and MCHC. When compared with normal control group, there is microcytic hypochromic anemia associated with leukocytosis, neutrophilia, eosinophilia, and lymphopenia, also thrombocytopenia is recorded. All these parameters were improved in treated (licorice and silymarin) CP intoxicated groups compared with non-treated CP intoxicated group.
The findings of this study showed that, administration of licorice and silymarin are effective when taken orally for 6 weeks, they alleviate the changes recorded in the hematological parameters induced by cisplatin injection.

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