Ezzat, M., ibrahim, M., ELgedway, A., ELTarabili, R., arnout, M. (2022). Prevalence, phenotypic characterization of listeria monocytogenes isolated from diseased sheep. Suez Canal Veterinary Medical Journal. SCVMJ, (), -. doi: 10.21608/scvmj.2022.145088.1087
Ezzat, M., ibrahim, M., ELgedway, A., ELTarabili, R., arnout, M. (2022). 'Prevalence, phenotypic characterization of listeria monocytogenes isolated from diseased sheep', Suez Canal Veterinary Medical Journal. SCVMJ, (), pp. -. doi: 10.21608/scvmj.2022.145088.1087
Ezzat, M., ibrahim, M., ELgedway, A., ELTarabili, R., arnout, M. Prevalence, phenotypic characterization of listeria monocytogenes isolated from diseased sheep. Suez Canal Veterinary Medical Journal. SCVMJ, 2022; (): -. doi: 10.21608/scvmj.2022.145088.1087
Prevalence, phenotypic characterization of listeria monocytogenes isolated from diseased sheep
Articles in Press, Accepted Manuscript, Available Online from 10 August 2022
1Professor of Microbiology, Faculty of Veterinary Medicine, Suez Canal University
2microbiology department,faculty of veterinary medicine,Zagazig university
3Microbiology Department, Animal Health Research Institute ,Dokki ,Egypt
4bacteriology,immunoloy and mycology department, faculty of veterinary medicine , Suez Canal University, Ismailia, Egypt
5veterinary quarintine,Cairo airport
Abstract
Listeriosis is the third most common foodborne illness and it is considered one of the most dangerous bacterial zoonotic infections globally. This research aimed to isolate and characterize Listeria monocytogenes (L. monocytogenes) from diseased sheep in Egypt. A total of 240 samples collected aseptically from sheep were subjected to isolation and biochemical characterization of L. monocytogenes. L. monocytogenes, which are Gram-positive rods that are both aerobic and facultatively anaerobic. Thirty-one listeria isolates (12.9%) were recovered from diseased sheep. All tested isolates were positive for catalase, Voges-Proskauer, carbohydrate fermentation, esculin, gelatin hydrolysis and methyl red tests. Meanwhile, they were negative for oxidase, indole, urease and nitrate reduction tests and they did not utilize citrate in the citrate utilization test. Listeria isolates produced yellow butt/yellow slant on TSI agar medium with no H2S or gas production. Therefore, this work focused on isolation of L. monocytgenes from diseased sheep and biochemical characterization of the recovered isolates using traditional and Microbact™ 12L listeria identification system.