Studies on Vibriosis in Solea Fish

Articles in Press

Document Type : Original Article

Authors

1 Fish managements and diseases,veterinary medicine, suez Canal university

2 Fish management and diseases department, veterinary medicine, Suez Canal university, ismailia

3 head researcher of bacteriology, Animal Health Research Institute, port said

4 Dept. of Fish diseases and Management, Fac. of Vet. Medecine, Suez Canal Univ.

Abstract

In the present study, a total of 120 Egyptian sole (Solea aegyptiaca) with different body weights (50-70 g) and lengths (13-19 cm) were collected randomly and seasonally from costal area of Mediterranean-sea in Port Said Governorate, Egypt from September-2019 to August-2020. The collected specimens whether moribund or freshly dead in aerated airbags and/or iceboxes were transferred to Food Hygiene and Bacteriology Unit, Animal Health Research Institute, Port Said for further bacteriological, and molecular examinations in particular for Vibrio infection. Fish were found to be infected with Vibrio alginolyticus based on the characteristic phenotypic and biochemical profiles with a total prevalence of 29.16%. The highest prevalence of vibriosis among the naturally infected fish was recorded in summer season (31.43%), followed by spring and winter (25.72%, for each), while the lowest prevalence of infection was recorded in the autumn (17.14%). Molecular investigation of pure isolates using specific set of primers targeting the 16S rRNA conserved gene of Vibrio species gave expected amplicons size of 663 bp.

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Suez Canal Veterinary Medical Journal. SCVMJ

Articles in Press, Accepted Manuscript
Available Online from 03 April 2023

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