Sameh, E., Elhady, K., Srour, S., El-Tarabili, R., Eltahan, A., Abdou, R. (2023). Comparative Effect of Stevia and Aspartame Sweeteners: A Biochemical, Sero-Biochemical, Histochemical and Immunohistochemical Study. Suez Canal Veterinary Medical Journal. SCVMJ, 28(1), 115-131. doi: 10.21608/scvmj.2023.307764
Eman Sameh; kawther Elhady; Sahar M. Srour; Reham El-Tarabili; Ahmed Shaker Eltahan; Rania H. Abdou. "Comparative Effect of Stevia and Aspartame Sweeteners: A Biochemical, Sero-Biochemical, Histochemical and Immunohistochemical Study". Suez Canal Veterinary Medical Journal. SCVMJ, 28, 1, 2023, 115-131. doi: 10.21608/scvmj.2023.307764
Sameh, E., Elhady, K., Srour, S., El-Tarabili, R., Eltahan, A., Abdou, R. (2023). 'Comparative Effect of Stevia and Aspartame Sweeteners: A Biochemical, Sero-Biochemical, Histochemical and Immunohistochemical Study', Suez Canal Veterinary Medical Journal. SCVMJ, 28(1), pp. 115-131. doi: 10.21608/scvmj.2023.307764
Sameh, E., Elhady, K., Srour, S., El-Tarabili, R., Eltahan, A., Abdou, R. Comparative Effect of Stevia and Aspartame Sweeteners: A Biochemical, Sero-Biochemical, Histochemical and Immunohistochemical Study. Suez Canal Veterinary Medical Journal. SCVMJ, 2023; 28(1): 115-131. doi: 10.21608/scvmj.2023.307764
Comparative Effect of Stevia and Aspartame Sweeteners: A Biochemical, Sero-Biochemical, Histochemical and Immunohistochemical Study
22Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine,Suez Canal University, Ismailia 41522, Egypt
3Biochemistry and Toxicology Department, Animal Health Research, Institute
4Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
5Department of Pathology, Faculty of Veterinary Medicine,Suez Canal University, Ismailia 41522, Egypt
Abstract
Aspartame is a common artificial sweetener that is thought to be dangerous. On the other hand, stevioside (stevia) a natural sweetener of plant origin was assumed to be a natural alternative to artificial sweeteners. This study aimed to determine whether aspartame or stevioside affected biochemical, serum-biochemical, and immunohistochemical changes in adult rats following six weeks of exposure. Forty-eight rats were used and divided into three groups: Group I: the control group, Group II: rats received aspartame (75 mg/kg/day), Group III: rats received stevioside (45 mg/kg/day). All medications were given orally for 6 weeks. Lipid Peroxidation product (MDA); liver glutathione (GSH); nitric oxide (NO); Liver Superoxidase dismutase activity(SOD); catalase (CAT), Low-density lipoprotein (LDL); very low lipoprotein (VLDL); the cholesterol in the HDL; Alanine Aminotransferase (ALT), and Aspartate Aminotransferase (AST); Creatinine, Urea, Total proteins were assessed in blood and liver tissue specimens. In addition, hepatic histopathological and immunohistochemical investigations were done. The Aspartame group showed a significant increase in most of the investigated biochemical parameters (especially glucose, MDA, liver NO, LDL, VLDL, ALT, AST, creatinine, and urea) with a significant decrease in serum albumin and the endogenous antioxidants (GSH, SOD, CAT) compared to the control group while stevia-exposed animals showed a significant decrease in the same biochemical parameters with a significant increase in the same endogenous antioxidants. Moreover, severe histopathological and immunohistochemical changes were observed in the liver of Aspartame- treated animals. However, Stevia-treated rats showed the least changes. Therefore, it can be concluded that Stevia was less harmful than aspartame.
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