The Antibacterial and Immunomodulatory Effect of Zinc-Oxide Nanoparticles on Pseudomonas aeruginosa Infected Fresh-Water Fish

Eldemerdash, Eman; Khalil, Waleed; Mehessen, Nada Fayez; Abdel-Fattah, Abdel-Fattah Mohamed

doi:10.21608/scvmj.2023.320242

The Antibacterial and Immunomodulatory Effect of Zinc-Oxide Nanoparticles on Pseudomonas aeruginosa Infected Fresh-Water Fish

Document Type : Original Article

Authors

¹ Free Veterinarian

² Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt

³ AssiPharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egyptstant Lecturer of Pharmacology

10.21608/scvmj.2023.320242

Abstract

In recent decades, Zinc oxide nanoparticles (ZnONPs) are already used in several fields either for commercial or industrial purposes. In this study, 120 freshwater fish (Oreochromis niloticus) were divided into 4 different groups, each in triplicate groups (n=10), and used to assess the pharmacological activity of ZnONPs as an anti-bacterial and immunomodulatory agent. Initially, the antibacterial activity was detected in-vitro through the disk diffusion method. The results revealed a potent antibacterial activity, especially at 80 mg/kg of ZnONPs. Then, Pathogenicity test with pseudomonas aeruginosa was done. The clinical signs following the infection were also detected and the mortality rate was calculated. Administration of ZnONPs (80mg/kg) showed potent ability to protect ‎the fish from mortality induced by I/P injection of P. aeruginosa. Finally,blood samples were collected twice, on the 20^th and 40^th days, to detect the effect of ZnONPs on some immune parameters including granulocyte, monocytes, lymphocytes and white blood cells (WBCs) count. The oral administration of both ZnONPs and zinc acetate resulted in a significant increase in lymphocyte, monocyte and WBCs count from the 20^th to 40^th days in ‎infected tilapia. In contrast, ‎ the results of granulocyte count revealed that the counts decreased from 20^th to 40^th days in groups exposed to 80mg/kg ZnO NPs. In conclusion, ZnO-NPs showed significant antibacterial activity against P. aeruginosa-infected tilapia fish either in-vitro or in-vivo as well as elevated the immunity of the fish against P. aeruginosa infection.

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