Histological and Immunohistochemical Alterations of Spinal Cord and Sciatic Nerve in Streptozotocin-Diabetic Rats

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Document Type : Original Article

Authors

1 Cytology and Histology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt

2 Prof. of Cytology and Histology, Faculty of Vet. Med., Suez Canal Univ.

3 Department of Physiology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

4 Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

5 Professor of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University

Abstract

The metabolic disorder; Diabetes Mellitus (DM) is the major origin of diabetic neuropathy (DN), which effects on the quality of life. Thus, we investigated the impact of diabetes mellitus on the sciatic nerve and spinal cord histology in streptozotocin (STZ)-induced diabetic rats. Twenty adult females Wistar rats were housed under standard condition, and then they divided into two groups; control and diabetic groups (10 rats each). Histological investigation of the sciatic nerve and spinal cord was performed by light microscopy and transmission electron microscopy. Also, immunohistochemical staining with S100 and GFAP were used. The histological and ultrastructural analysis of spinal cord and sciatic nerve revealed sever changes of diabetic group in comparison to control one.
Moreover, a significant decrease in the immunostaining expression of S100 and GFAP in spinal cord of diabetic rats. The decline of the staining affinity was observed in S100 stain of sciatic nerve of diabetic rats.

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Suez Canal Veterinary Medical Journal. SCVMJ

Articles in Press, Accepted Manuscript
Available Online from 16 July 2024

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