Molecular Characterization of Salmonella Enterica Serovars Isolated From Chicken

Document Type : Original Article

Authors

1 Department of Microbiology, Faculty of Veterinary Medicine, Suez Canal University

2 Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University

3 Administration of veterinary medicine, Sharkia Governorate, Zagazig,

4 Animal Health Research institute, Zagazig, Egypt.

Abstract

The estimation of virulence and antibiotic resistance in Salmonella strains from chickens helps in mapping out suitable preventive ways to control the spread of this enteric microorganism in Egypt. Therefore this work was designed to study the molecular characterization of Salmonella enterica serovars isolated from different samples in chicken. Two hundred and seventy samples collected from different farms at Sharkia Governorate during the period from November 2015 to May 2016. All Salmonella isolates were analyzed for the presence of Salmonella serovars and for the presence of the most predominant virulence genes. The result revealed an incidence of Salmonella from faecal swabs and internal organs (38/270) with an overall incidence 14.07% from chicks only. Additionally, absolute resistance was observed for amoxicillin/clavulanic acid, ampicillin, doxycycline and ceftriaxone. On the other hand, sensitivity to ciprofloxacin (81.6%), gentamicin (78.9%) , sulphamethoxazol/trimethoprim (73.7%) ,amoxicillin (65.8%)and chloramphenicol(63.2%). All isolates of Salmonella were positive to invA ,hilA , avrA, bcfC, stn and sop < /em>B genes but  pefA gene found only 1 sample  (12.5%) . Combining between the phenotypic antimicrobial results and PCR pathotyping of different Salmonella spp. may be effective in providing a more accurate profile to understand the danger of spread of different virulence genes and multidrug resistance in Salmonella spp.
 

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Suez Canal Veterinary Medical Journal. SCVMJ
Volume 22, Issue 2
December 2017
Pages 19-32

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